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KMID : 0380220070400050839
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Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 5 p.839 ~ p.843
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Backbone 1H, 15N, and 13C Resonance Assignment and Secondary Structure Prediction of HP0495 from Helicobacter pylori
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Seo Min-Duk
Park Sung-Jean
Kim Hyun-Jung
Seok Seung-Hyeon
Lee Bong-Jin
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Abstract
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HP0495 (Swiss-Prot ID; Y495_HELPY) is an 86-residue hypothetical protein from Helicobacter pylori strain 26695. The function of HP0495 cannot be identified based on sequence homology, and HP0495 is included in a fairly unique sequence family. Here, we report the sequencespecific backbone resonance assignments of HP0495. About 97% of all the 1HN, 15N, 13C&agr;, 13C&bgr;, and 13CO resonances were assigned unambiguously. We could predict the secondary structure of HP0495, by analyzing the deviation of the 13C&agr; and 13C&bgr; shemical shifts from their respective random coil values. Secondary structure prediction shows that HP0495 consists of two &agr;-helices and four &bgr;-strands. This study is a prerequisite for determining the solution structure of HP0495 and investigating the protein-protein interaction between HP0495 and other Helicobacter pylori proteins.
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KEYWORD
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Backbone assignment, HP0495, NMR, Secondary structure
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